Generation and Utilization of Transgenic Mice

All mice used in this study were C57BL/6, or derived from a C57BL/6 background. Mice were housed in specific pathogen-free conditions. CD4-Cre (Lee et al., 2001 (link)) and homozygous iDTR (Buch et al., 2005 (link)) breeding pairs were purchased from Jackson Laboratory, and were crossed to generate CD4-Cre/iDTR, referred to as CD4^DTR, mice (Buch et al., 2005 (link)). Systemic CD4 T-cell ablation in the CD4^DTR mice was achieved by an initial intraperitoneal injection of 1µg diphtheria toxin (DT; Sigma) and an additional 200ng DT every 4 days. Tbet-zsGreen mice (Zhu et al., 2012 (link)) were crossed with FoxP3-RFP mice (Wan and Flavell, 2005 (link)) to generate Tbet-zsGreen FoxP3-RFP mice and were kindly gifted by Marc Jenkins. Mice used for all experiments were 6-8 weeks of age; all controls were sex- and age-matched.