In-vitro Evaluation of HMG-CoA Reductase and PL

The effect of the EA fraction on the activities of HMG-CoA reductase and PL was tested in vitro. The assay of the activity of HMG-CoA reductase is based on NADPH oxidation by the catalytic subunit of HMG reductase in the presence of HMG-CoA (Rao and Ramakrishnan, 1975 (link)) using pravastatin as a standard inhibitor. PL activity was measured using 4-methyl umbelliferone oleate (4 MUO) as a substrate. Using microplate reader at an excitation wavelength of 320 nm and an emission wavelength of 450 nm, the amount of 4-MUO liberated by lipase was measured.

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Abduh M.S., Saghir S.A., Al Hroob A.M., Bin-Ammar A., Al-Tarawni A.H., Murugaiyah V, & Mahmoud A.M. (2023). Averrhoa carambola leaves prevent dyslipidemia and oxidative stress in a rat model of poloxamer-407-induced acute hyperlipidemia. Frontiers in Pharmacology, 14, 1134812.

Publication 2023

Assay Catalytic subunit Hmg coa Hmg coa reductase Lipase Methyl oleate Nadph Pravastatin Reductase Umbelliferone

Corresponding Organization : Beni-Suef University

Other organizations : University of Ha'il, Mutah University

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Variable analysis

independent variables

EA fraction

dependent variables

Activity of HMG-CoA reductase
Activity of PL

control variables

Presence of HMG-CoA
Presence of 4-methyl umbelliferone oleate (4 MUO) as a substrate for PL activity

controls

Pravastatin as a standard inhibitor for HMG-CoA reductase activity

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