Cell Lines for Cancer Research

The human lung cancer cell line H460, and its MX-selected derivative BCRP-overexpressing cell line, H460/MX20, were used in this study. The KB-C2 cell line was selected by colchicine (Alfa Aesar, Haverhill, MA, USA) (2 μg/mL) with human cervical carcinoma cell line KB-3-1. All the cell lines were maintained in Dulbecco’s Modified Eagle Medium (Corning Inc., New York, NY, USA) containing 10% fetal bovine serum (Gibco Inc., Billings, MT, USA) and 1% penicillin/streptomycin (Gibco Inc.) at 37 °C with 5% CO₂.

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Cai C.Y., Teng Q.X., Murakami M., Ambudkar S.V., Chen Z.S, & Korlipara V.L. (2023). Design, Synthesis and Biological Evaluation of Quinazolinamine Derivatives as Breast Cancer Resistance Protein and P-Glycoprotein Inhibitors with Improved Metabolic Stability. Biomolecules, 13(2), 253.

Publication 2023

colchicine Dulbecco’s Modified Eagle Medium fetal bovine serum penicillin/streptomycin

Carcinoma Cell Cell lines Cervical Colchicine Eagle Fetal bovine serum Human Kb cell Line 1 Lung cancer Penicillin Streptomycin

Corresponding Organization :

Other organizations : St. John's University, National Cancer Institute, National Institutes of Health, Center for Cancer Research

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Variable analysis

independent variables

Selection of the H460/MX20 cell line by MX (Alfa Aesar, Haverhill, MA, USA) (2 μg/mL)
Selection of the KB-C2 cell line by colchicine (Alfa Aesar, Haverhill, MA, USA) (2 μg/mL)

dependent variables

Not explicitly mentioned

control variables

Cell culture conditions: Dulbecco's Modified Eagle Medium (Corning Inc., New York, NY, USA) containing 10% fetal bovine serum (Gibco Inc., Billings, MT, USA) and 1% penicillin/streptomycin (Gibco Inc.) at 37 °C with 5% CO2

controls

Positive control: Human lung cancer cell line H460
Negative control: Not explicitly mentioned

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