HIF-1α Inhibition in Hypoxia

To inhibit the expression of HIF-1α, the specific inhibitor of HIF-1α (2-MeOE2), which was purchased from Selleck Chemicals LLC, was added into the induced culture medium for 24 h in hypoxic conditions, as described previously [35 (link)]. A concentration gradient test was performed to determine the minimum effective concentration (MEC) of the inhibitor. The 2-methoxyestradiol was used at concentration of 0, 5, 10, and 15 µM, respectively. ADSCs were then treated with 2-methoxyestradiol at MEC in both normoxia and hypoxia samples.

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Guo X., Huang D., Li D., Zou L., Lv H., Wang Y, & Tan M. (2022). Adipose-derived mesenchymal stem cells with hypoxic preconditioning improve tenogenic differentiation. Journal of Orthopaedic Surgery and Research, 17, 49.

Publication 2022

2-MeOE2

2 methoxyestradiol Culture medium H conditions Hypoxia Inhibit

Corresponding Organization :

Other organizations : Affiliated Hospital of Southwest Medical University

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Variable analysis

independent variables

Concentration of the HIF-1α inhibitor 2-MeOE2 (0, 5, 10, and 15 µM)

dependent variables

Expression of HIF-1α

control variables

Culture conditions (normoxia and hypoxia)
Cell type (ADSCs)

controls

Positive control: Untreated ADSCs cultured under hypoxic conditions to induce HIF-1α expression
Negative control: Untreated ADSCs cultured under normoxic conditions

Annotations

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