After 60 days of the ovariectomy (OVX) and simulated ovariectomy (Sham) procedures, the same rats were subjected to the manufacture of critical bone defects in the calvaria. The animals were weighed and anesthetized with a solution of xylazine hydrochloride 2.3 g/100 mL (Anasedan®, Vetbrands, Jacareí, Brazil) and ketamine hydrochloride 1.16 g/10 mL (Dopalen®, Vetbrands, Jacareí, Brazil).
After the anesthesia of the animals, the surgical sites were subjected to trichotomy and antisepsis with an iodized alcohol solution. A linear incision of approximately 3.5 mm was performed with a scalpel blade number 15 in the region corresponding to the medial face of the calvaria. The tissues were divulsed to expose the calvaria cortices, in which the 5.0 mm defects were made under abundant and continuous irrigation with physiological solution to prevent heating due to the friction of the drill with the bone. To obtain the critical defect, a 5.0 mm diameter drill was used. The defects on the left side in the OVX group were made with (a) bioglass (BG) or (b) bioglass associated with 10% teriparatide (BGT), and on right side, they were filled with clot. In the sham group, the defect was filled only by clot. For the stabilization of the material in the surgical site of the defect, a larger-diameter biological membrane (GenDerm®) was positioned at the bone defect site. In all defects, the tissues were repositioned, and the layers were sutured with no3 silk thread (Ethicon/Johnson & Johnson). Again, iodized alcohol antisepsis was performed. After surgery, the rats were placed in mini-isolate of the ventilated rack and monitored until the euthanasia deadline.
After the anesthesia of the animals, the surgical sites were subjected to trichotomy and antisepsis with an iodized alcohol solution. A linear incision of approximately 3.5 mm was performed with a scalpel blade number 15 in the region corresponding to the medial face of the calvaria. The tissues were divulsed to expose the calvaria cortices, in which the 5.0 mm defects were made under abundant and continuous irrigation with physiological solution to prevent heating due to the friction of the drill with the bone. To obtain the critical defect, a 5.0 mm diameter drill was used. The defects on the left side in the OVX group were made with (a) bioglass (BG) or (b) bioglass associated with 10% teriparatide (BGT), and on right side, they were filled with clot. In the sham group, the defect was filled only by clot. For the stabilization of the material in the surgical site of the defect, a larger-diameter biological membrane (GenDerm®) was positioned at the bone defect site. In all defects, the tissues were repositioned, and the layers were sutured with no3 silk thread (Ethicon/Johnson & Johnson). Again, iodized alcohol antisepsis was performed. After surgery, the rats were placed in mini-isolate of the ventilated rack and monitored until the euthanasia deadline.
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