Cardiac Fibroblast Progenitor Isolation

Steps taken to isolate cardiac FAPs are shown in Online Figure I. In brief, non-cardiac myocyte fraction of heart cells was sorted to isolate cells that express PDGFRA and exclude those expressing hematopoietic lineage markers CD32, CD11B, CD45, Lys76, Ly^−6c and Ly^6c (Lin^neg), stem cell and fibroblast marker THY1, and fibroblast marker DDR2. The isolated mouse PDGFRA^pos:Lin^neg:THY1^neg:DDR2^neg cells were also analyzed for the expression of lineage markers TIE2, PDGFRB, CD146, and KIT antigen by flow cytometry and/or immunostaining.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Lombardi R., Chen S.N., Ruggiero A., Gurha P., Czernuszewicz G.Z., Willerson J.T, & Marian A.J. (2016). Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene. Circulation research, 119(1), 41-54.

Publication 2016

Antigen Cardiac myocyte Cd11b Cells Faps Fibroblast Flow cytometry Heart Hematopoietic Mouse Stem cell

Corresponding Organization :

Other organizations : Houston Methodist, The University of Texas Health Science Center at Houston

Top 5 similar protocols

Protocol cited in 4 other protocols

Variable analysis

independent variables

Sorting non-cardiac myocyte fraction of heart cells to isolate cells that express PDGFRA and exclude those expressing hematopoietic lineage markers CD32, CD11B, CD45, Lys76, Ly-6c and Ly6c (Lin-neg), stem cell and fibroblast marker THY1, and fibroblast marker DDR2

dependent variables

Expression of lineage markers TIE2, PDGFRB, CD146, and KIT antigen in the isolated mouse PDGFRA-pos:Lin-neg:THY1-neg:DDR2-neg cells, measured by flow cytometry and/or immunostaining

control variables

Independent variables not explicitly mentioned.
Dependent variables not explicitly mentioned.
Control variables not explicitly mentioned.

positive controls

None specified.

negative controls

None specified.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!