Artificial cerebrospinal fluid (aCSF; 145 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 1.2 mM CaCl2, 0.45 mM monobasic phosphate, 1.55 mM dibasic phosphate, pH 7.4) and high potassium aCSF (95 mM NaCl, 50 mM KCl, 1.0 mM MgCl2, 1.2 mM CaCl2, 0.45 mM monobasic phosphate, 1.55 mM dibasic phosphate, pH 7.4) was prepared weekly with components from Fisher Scientific (Pittsburgh, PA). Ro60-0175 and ketanserin were purchased from Tocris Bioscience (Bristol UK). The novel compounds, (−)-trans-4-phenyl-2-N,N-dimethylaminotetralin ((−)-trans- PAT), (−)-trans-4-(4′ [para]-chlorophenyl)-2-N,N-dimethylaminotetralin ((−)-trans-p-Cl-PAT), (−)- trans-4-cylcohexyl-2-N,N-dimethylaminotetralin ((−)-trans-CAT), and (−)-trans-4-(3′ [meta]-chlorphenyl)-6-methoxy-N,N-dimethyl-1,2,3,4-tetrahydronaphthalene-2-amine (m-Cl-6-OMe- PAT) were synthesized in the University of Florida Department of Medicinal Chemistry laboratories with details reported elsewhere (Booth et al., 2009 (link); Canal et al., 2013b (link); Morgan et al., 2013 (link); Vincek and Booth, 2009 (link)). All drugs were prepared in aCSF.
Protocol detail
Synthetic Cerebrospinal Fluid Preparation
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Corresponding Organization :
Other organizations : The University of Texas Medical Branch at Galveston, Northeastern University, University of Florida
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Variable analysis
independent variables
- Ro60-0175
- Ketanserin
- (−)-trans-PAT
- (−)-trans-p-Cl-PAT
- (−)-trans-CAT
- M-Cl-6-OMe-PAT
- Unspecified
- Artificial cerebrospinal fluid (aCSF; 145 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 1.2 mM CaCl2, 0.45 mM monobasic phosphate, 1.55 mM dibasic phosphate, pH 7.4)
- High potassium aCSF (95 mM NaCl, 50 mM KCl, 1.0 mM MgCl2, 1.2 mM CaCl2, 0.45 mM monobasic phosphate, 1.55 mM dibasic phosphate, pH 7.4)
- Artificial cerebrospinal fluid (aCSF; 145 mM NaCl, 2.7 mM KCl, 1.0 mM MgCl2, 1.2 mM CaCl2, 0.45 mM monobasic phosphate, 1.55 mM dibasic phosphate, pH 7.4)
- High potassium aCSF (95 mM NaCl, 50 mM KCl, 1.0 mM MgCl2, 1.2 mM CaCl2, 0.45 mM monobasic phosphate, 1.55 mM dibasic phosphate, pH 7.4)
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