OCT Detection of Glioblastoma in Mice

To test the ability of OCT to detect cancer from non-cancer in vivo, five, 8-week old, NOD/SCID male mice (Charles River Laboratories) were stereotactically inoculated with cancer cells as previously described (38 ), but with coordinates X: 3.5, Y: 1.4 and Z: 3.5 based on bregma. In three mice, we injected 10⁶ glioblastoma cells from a commercial U87 cell line (ATCC^®), which produces mostly spherical brain tumors; in two mice, we injected 10⁶ glioblastoma cells from a patient-derived primary stem cell line (GBM272) which is slightly more infiltrative and better replicates the migratory and invasive behavior of cancer cells in glioblastoma patients (fig. S4) (39 (link), 40 (link)). The mice underwent cancer resection (38 ) at 4 weeks post implantation, and OCT images were taken as described in Supplementary Methods (fig. S5).