Total protein lysates or membrane-enriched protein extracts were prepared, separated by SDS-PAGE, and transferred onto a polyvinylidene difluoride membrane for Western blotting as described previously [54 (link)]. Primary antibodies used are given in Supplementary Table S6. Secondary, horseradish peroxidase-labeled antibodies from Santa Cruz Biotechnology were used in working dilutions of 1:10 000.
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