Quantification and visualization of marrow adipose tissue was performed as described in (Scheller et al. 2014 (link)). Briefly, long bones were dissected free of soft tissues and fixed in 10% neutral buffered formalin (cat# SF100-4, Fisher Scientific, Pittsburgh, PA, USA) overnight at 4°C with gentle agitation. The following day, bones were washed in cool running tap water. The bones were then decalcified in 4% EDTA for 14 days at 4°C, with EDTA changes every 3-4 days. The bones were then stained for lipid using a 1:1 mixture of 2% aqueous osmium tetroxide (cat# 23310-10, Polysciences Inc., Warrington, PA) and 5% potassium dichromate for 48 hours. The bones were then washed in cool running tap water for 2 hours. Whole bones were imaged using micro-computed tomography (μCT) performed in water with energy of 55kVp, an integration time of 500 ms, and a maximum isometric voxel size of 10μm using a μCT-35 (Scanco Medical, Bruttisellen, Switzerland).