In vivo OCT Imaging of Griseofulvin-Injected Mice

In vivo OCT was performed in griseofulvin injected adult mice as described previously (39 (link), 42 (link)) at 5 and 14 days after injection using the Micron III intraocular imaging system (Phoenix Research Laboratories, Pleasanton, CA, USA). Briefly, before the procedure, mice were anesthetized with ketamine (90 mg/kg) and xylazine (5 mg/kg) and their pupils were dilated with 1% tropicamide solution (Alcon, Fort Worth, TX, USA) and lubricated with hypromellose ophthalmic demulcent solution (Gonak; Akorn, Lake Forest, IL, USA). Mice were then placed on a custom heated stage that moved freely to position the mouse eye for imaging. Several horizontal and vertical OCT images were taken in untouched, vehicle and griseofulvin-injected mice.