Immunohistochemical Analysis of Neuronal Signaling

The antibodies and reagents used in this study were as follows: GFP (A6455; rabbit; Invitrogen; Chen et al., 2011 (link)); MAP2 (AB5622; rabbit; EMD Millipore; Chen et al., 2011 (link)); MAP2 (M4403; mouse; Sigma-Aldrich; Chen et al., 2011 (link)); SMI-312R (SMI-312R; mouse; Covance; Liu et al., 2013 (link)); HA (3F10; rat; Roche; Chen et al., 2017 (link)); phospho-P38 MAPK (9211; rabbit; Cell Signaling Technology); rabbit polyclonal P38 MAPK antibody (9212; rabbit; Cell Signaling Technology); phospho-ERK (4376; rabbit; Cell Signaling Technology); ERK (4695; rabbit; Cell Signaling Technology); phospho-JNK (9251; rabbit; Cell Signaling Technology); JNK (9252; rabbit; Cell Signaling Technology); phospho-TAK1 (9339; rabbit; Cell Signaling Technology); GAPDH (sc-25778; rabbit; Santa Cruz Biotechnology, Inc.; Chen et al., 2011 (link)); NeuN (MAB377; mouse; EMD Millipore; Wang et al., 2015 (link)); HRP-conjugated secondary antibodies (GE Healthcare); and Alexa Fluor 488– and Alexa Fluor 594–conjugated secondary antibodies (Invitrogen). Antibodies with validation profiles in Antibodypedia or 1DegreeBio are underlined. CL075, poly dT, poly(I:C) high molecular weight, and SB203580 were all purchased from InvivoGen. Takinib and NG 25 were purchased from Medchem Express, and (5Z)-7-oxozeaenol was purchased from Tocris.

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Hung Y.F., Chen C.Y., Shih Y.C., Liu H.Y., Huang C.M, & Hsueh Y.P. (2018). Endosomal TLR3, TLR7, and TLR8 control neuronal morphology through different transcriptional programs. The Journal of Cell Biology, 217(8), 2727-2742.

Publication 2018

GFP (A6455; SMI-312R HA (3F10; phospho-P38 MAPK (9211; ERK (4695; phospho-JNK (9251 JNK (9252 GAPDH (sc-25778 NeuN HRP-conjugated secondary antibodies Alexa Fluor 488– and Alexa Fluor 594–conjugated secondary antibodies CL075 poly dT poly(I:C) high molecular weight SB203580 Takinib (5Z)-7-oxozeaenol

7 oxozeaenol Alexa 594 Alexa fluor 488 Antibodies Antibody Gapdh Map2 Mouse P38 mapk Poly dt Poly i c Rabbit Sb203580 Takinib

Corresponding Organization : Institute of Molecular Biology, Academia Sinica

Other organizations : National Yang Ming Chiao Tung University

Top 5 similar protocols

Variable analysis

independent variables

CL075
Poly dT
Poly(I:C) high molecular weight
SB203580
Takinib
NG 25
(5Z)-7-oxozeaenol

dependent variables

Phospho-P38 MAPK
P38 MAPK
Phospho-ERK
Phospho-JNK
Phospho-TAK1

control variables

SMI-312R
GAPDH
HRP-conjugated secondary antibodies
Alexa Fluor 488– and Alexa Fluor 594–conjugated secondary antibodies

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