Transfection and miCLIP Analysis of NSun2 Mutant
Corresponding Organization :
Other organizations : University of Cambridge, Wellcome/MRC Cambridge Stem Cell Institute, Medical Research Council, Centre National de la Recherche Scientifique, Johannes Gutenberg University Mainz, Helmholtz Zentrum München, German Center for Lung Research, Ludwig-Maximilians-Universität München, Friedrich Baur Stiftung, University College London, Howard Hughes Medical Institute, Rockefeller University
Variable analysis
- Myc-tagged NSun2 C271A mutated construct
- Empty vector control
- MiCLIP (molecular identification of crosslinked and immunoprecipitated RNA)
- Human fibroblasts
- Amaxa nucleofection kit (Lonza)
- Cells harvested 48 h later
- Empty vector control
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