Endothelial Cell Culture for Vascular Research

Primary human umbilical vein endothelial cells (HUVEC) were purchased from Cell Applications (200K-05), primary human saphenous vein endothelial cells (HSaVEC) and human aortic endothelial cells (HAoEC) from PromoCell (C-12231 and C-12271, respectively) and human coronary arterial endothelial cells (HCoEC) from Lonza (CC-2585). All cells were cultured in Microvascular Endothelial Cell Growth Medium-2 (complete medium, EGM-2MV, Lonza) and used for no more than 5 passages (16 (link)).

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Maier-Begandt D., Comstra H.S., Molina S.A., Krüger N., Ruddiman C.A., Chen Y.L., Chen X., Biwer L.A., Johnstone S.R., Lohman A., Good M.E., DeLalio L.J., Hong K., Bacon H., Yan Z., Sonkusare S.K., Koval M, & Isakson B.E. (2021). A venous-specific purinergic signaling cascade initiated by Pannexin 1 regulates TNFα-induced increases in endothelial permeability. Science signaling, 14(672), eaba2940.

Publication 2021

HAoEC EGM-2MV

Aortic Cells Coronary arterial Cultured cell Endothelial Endothelial cells Human Human umbilical vein endothelial cells Saphenous vein

Corresponding Organization : Emory University

Other organizations : Ludwig-Maximilians-Universität München, Heinrich Heine University Düsseldorf, Virginia Tech, Carilion Roanoke Memorial Hospital, University of Calgary, Tufts Medical Center, Chung-Ang University

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Variable analysis

independent variables

Cell type

dependent variables

Not explicitly mentioned

control variables

Passage number (used for no more than 5 passages)
Culture medium (Microvascular Endothelial Cell Growth Medium-2)

controls

No positive or negative controls were explicitly mentioned

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