HTM cells were cultured in collagen-coated plates (Flexcell International, Burlington, USA, BioFlex plates, BF-3001C), and after 90% confluency, the plates were fixed on a FlexCell (Flexcell International, FX-6000 Tension System). The cells were stretched at 0.69 Hz frequency with 15% stretching for 8h for gene expression analysis and 24 h for protein expression analysis based on previously published report (Luna et al., 2009 (link)). Cells and media were collected and processed further.
Protocol detail
Mechanical Stretching of HTM Cells
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Indiana University – Purdue University Indianapolis
Other organizations : University School, Case Western Reserve University
Top 5 similar protocols
Variable analysis
independent variables
- Stretching frequency at 0.69 Hz
- Stretching percentage at 15%
- Gene expression analysis
- Protein expression analysis
- HTM cells cultured in collagen-coated plates
- Cells at 90% confluency
- Plates fixed on a FlexCell (Flexcell International, FX-6000 Tension System)
- Stretching duration of 8 hours for gene expression analysis and 24 hours for protein expression analysis
- No positive or negative controls were explicitly mentioned in the input protocol.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!