Generating CD11c+ Cell-Specific gp96-Deficient Mice

CD11c⁺ cell specific gp96-deficient mice (CD11cCre⁺Hsp90b1^flox/flox) and control littermates (CD11cCre⁻Hsp90b1^flox/flox) were generated by crossing our Hsp90b1^flox/flox (gp96 is encoded by Hsp90b1) mice^{36 (link)} with CD11c-cre transgenic mice^{59 (link)}. These gp96-deficient mice were further crossed with CX₃CR1-GFP transgenic mice^{46 (link)} (Jackson laboratory) to define CX₃CR1⁺ population. OT-II TCR transgenic mice were purchased from Jackson laboratory and bred onto the Ly5.1 background. All animal experimental protocols were approved by the Medical University of South Carolina Institutional Animal Care and Use Committee (IACUC). All methods were carried out in accordance with federal regulation as well as established institutional guidelines and regulations.