In vitro translation assays were performed as previously described [15 (link)]. 3 pmol of RNA transcripts were used in a 25 μL translation reaction using wheat germ extract (WGE) (Promega) according to the manufacturer′s instructions. The luciferase activity was measured using a luciferase assay reporter system (Promega) and a Modulus Microplate Multimode Reader (Turner BioSystems). At least three independent in vitro translationa ssays were performed for each construct. Standard errors were calculated in Microsoft Excel.
Free full text: Click here