Measuring Nucleotide Levels in Enzyme Reactions

eATP, eADP, eAMP and eADO in enzyme reaction solutions of nondistended and distended preparations were measured using a reverse-phased gradient Agilent Technologies 1200 liquid chromatography system equipped with a fluorescence detector (Agilent Technologies, Wilmington, DE, USA) as described previously [9 (link),34 (link),82 (link)]. Areas of HPLC peaks were calculated and calibrated to individual etheno-derivatized purine standards of eATP, eADP, eAMP and eADO.

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Aresta Branco M.S., Gutierrez Cruz A., Borhani Peikani M, & Mutafova-Yambolieva V.N. (2023). Sensory Neurons, PIEZO Channels and PAC1 Receptors Regulate the Mechanosensitive Release of Soluble Ectonucleotidases in the Murine Urinary Bladder Lamina Propria. International Journal of Molecular Sciences, 24(8), 7322.

Publication 2023

1200 liquid chromatography system fluorescence detector

Enzyme Fluorescence Hplc Purine Reverse phased liquid chromatography

Corresponding Organization : University of Nevada, Reno

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