In Vivo Imaging of Amyloid-β with [18F]-Florbetaben

Image acquisition and reconstruction followed a standardized protocol (Brendel et al., 2015b (link)). Mice were anesthetized with isoflurane (1.5%, delivered via a mask at 3.5 L/min in oxygen) and received bolus injection of 10.1 ± 2.3 MBq of [¹⁸F]-florbetaben in 150 μL of saline to a tail vein. Following placement in the tomograph (Siemens Inveon DPET), a single frame emission recording for the interval 30–60 min p.i. followed by a 15 min transmission scan was obtained using a rotating [⁵⁷Co] point source. The image reconstruction procedure consisted of an three-dimensional ordered subset expectation maximization (OSEM) with four iterations and 12 subsets followed by a maximum a posteriori (MAP) algorithm with 32 iterations. Scatter and attenuation correction were performed and a decay correction for [¹⁸F] was applied. With a zoom factor of 1.0 and a128 × 128 × 159 matrix, a final voxel dimension of 0.78 × 0.78 × 0.80 mm was obtained.