Gliding Assay for Parasite Motility

Gliding assays were performed as described before [5 (link)]. Briefly, freshly lysed parasites were allowed to glide on FBS-coated glass slides for 30 minutes before they were fixed with 4% paraformaldehyde (PFA) and stained with α-SAG1 under nonpermeabilising conditions. The mean values of three independent experiments ± SD were determined. Where drugs were used, parasites were pre-incubated for 10 minutes in the respective concentration before the start of the assay: 0.5 μM CD (Sigma, St. Louis, MO), 10 μM phenyl arsine oxide (Sigma, St. Louis, MO), or 50 μM trifluoperazine dihydrochloride (TFDC) (Sigma, St. Louis, MO). The same concentrations were used in the different assays.