Cell Viability Assay and Combination Analysis

The cell counting kit-8 assay (Dojindo Molecular Technologies) was carried out according to the manufacturer's guidelines and as previously described [37 (link)]. IC50s were calculated from sigmoidal dose-response curves utilizing Prism. The combination effect was determined by the combination index (CI) method [27 (link)] using the Calcusyn software program (Biosoft). Data from cell viability assays were expressed as the fraction of growth inhibition by the individual drugs or the combination in drug-treated cells. Synergism was indicated by a CI values less than 1 and antagonism by a CI value more than 1 at 50% effect (Fraction Affected = 0.5).

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Wang D., Wang M., Jiang N., Zhang Y., Bian X., Wang X., Roberts T.M., Zhao J.J., Liu P, & Cheng H. (2016). Effective use of PI3K inhibitor BKM120 and PARP inhibitor Olaparib to treat PIK3CA mutant ovarian cancer. Oncotarget, 7(11), 13153-13166.

Publication 2016

cell counting kit-8 assay Calcusyn software program

Antagonism Assays Cell viability Combination drug Drugs Inhibition Prism

Corresponding Organization :

Other organizations : Binzhou Medical University, Dalian Medical University, Dana-Farber Cancer Institute, Harvard University

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Variable analysis

independent variables

Dose of individual drugs
Combination of drugs

dependent variables

Cell viability
IC50 values
Combination index (CI)

control variables

Manufacturer's guidelines for the cell counting kit-8 assay
Previously described protocol [37]

controls

No positive or negative controls were explicitly mentioned.

Annotations

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