Probiotic Supplement Formulation and Preparation

Example 1

NAME OF COMPONENTmg/sachet

Probiotic Material:

Lactobacillus helveticus150 billion CFU/g73.333

Rosell 52

Bifidobacterium longum 50 billion CFU/g20.000

R175

Lactobacillus plantarum150 billion CFU/g20.000

Rosell 1012

Carrier material:

Magnesium oxide41.446

Magnesium gluconate341.297

Potassium citrate138.290

Zinc gluconate111.111

Glutathione20.000

Lactoferrin11.364

Copper citrate2.834

Inulin500.000

Fructose1291.125

Additional (optional) excipients

Sucralose4.000

Acesulfame K12.000

Flavouring150.000

Aerosil 20040.000

Colouring: E1242.200

Colouring: E1021.000

Anhydrous citric acid220.000

The formulation described above is prepared as follows: Lactobacillus Plantarum, Lactobacillus helveticus, Bifidobacterium longum, are mixed with inulin and blended at 32 rpm for approximately 10 min. Thereafter, fructose, magnesium gluconate, zinc gluconate, citric acid, flavor, potassium citrate, magnesium oxide, silicon dioxide, glutathione, potassium acesulfame, lactoferrine, and sucralose are added to the mixture and blended at 32 rpm for another 10 min.

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US11878040B2. Compositions comprising probiotic and prebiotic components and mineral salts, with lactoferrin (2024-01-23). GlaxoSmithKline Consumer Healthcare S.R.L. [RO]. Inventors: Valeria Longoni [IT], Marisa Penci [IT].

Patent 2024

Aerosil Anhydrous citric acid Bifidobacterium longum Citric acid Copper Excipients Flavor Fructose Gluconate Glutathione Inulin Lactobacillus Lactobacillus helveticus Lactobacillus plantarum Lactoferrin Magnesium gluconate Magnesium oxide Mg component Mineral Oxide Potassium acesulfame Potassium citrate Prebiotic Probiotic Salts Silicon dioxide Sucralose Zinc gluconate

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Variable analysis

independent variables

Mixing speed (32 rpm)
Mixing time (10 minutes)

dependent variables

Composition of the final formulation (amount of each ingredient)

control variables

Ingredients used (Lactobacillus Plantarum, Lactobacillus helveticus, Bifidobacterium longum, inulin, fructose, magnesium gluconate, zinc gluconate, citric acid, flavor, potassium citrate, magnesium oxide, silicon dioxide, glutathione, potassium acesulfame, lactoferrine, and sucralose)

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