Genetic Variants and Platinum-Based Chemotherapy Toxicity

All of the common genetic variants in MLH1, MSH2, MSH3, MSH4, MSH5, SAPCD1 and MSH6 were selected by Haploview (Broad Institute, Cambridge, MA, USA) using pair-wise tagging with default settings (pairwise r² threshold = 0.8). SNPs with a minor allele frequency (MAF) ≥ 5% in the Han Chinese population were selected. Finally, Thirty-five SNPs were genotyped in the patients (Table 1). In our previous studies, the SNPs were investigated to be associated with platinum-based chemotherapy toxicity 30 (link).
ALL blood samples were collected in the morning and stored in EDTA tube. Genomic DNA was isolated using a Genomic DNA Purification Kit (Promega, Madison, WI, USA) and stored at -20°C before use. Genotyping was analyzed using a Sequenom Mass ARRAY Genotyping Platform (Sequenom, San Diego, CA, USA) through polymerase chain reaction (PCR) system.