Protocol detail

Induction of Chronic Arthritis in Rodents

Find Similar Protocols

CFA-induced arthritis is a well-established rodent model of chronic arthritis [41 (link),42 (link),43 (link),44 (link)], and it was elicited according to our standard protocol [45 (link),46 (link)]. Inflammation of the right tibiotarsal joint was induced by subcutaneous injection of 20 µL of complete Freund’s adjuvant (CFA; consisting of heat-killed Mycobacterium tuberculosis suspended in paraffin oil, 1 mg/mL; Sigma Aldrich, St. Louis, MO, USA) into the plantar surface of the right hind paw and to the tail root under i.p. ketamine (100 mg/kg Calypsol, Gedeon Richter Plc., Budapest, Hungary) and xylazine (5 mg/kg Sedaxylan, Eurovet Animal Health B.V., Bladel, The Netherlands) anesthesia (on Day 0). An additional tail root injection was administered in the same volume on Day 1 to boost the systemic inflammatory effect.

Free full text: Click here

Rusznák K., Horváth Á.I., Pohli-Tóth K., Futácsi A., Kemény Á., Kiss G., Helyes Z, & Czéh B. (2022). Experimental Arthritis Inhibits Adult Hippocampal Neurogenesis in Mice. Cells, 11(5), 791.

Publication 2022

Mycobacterium tuberculosis

Anesthesia Animal Calypsol Freund adjuvant Inflammation of joint Inflammatory Ketamine Mycobacterium tuberculosis Paraffin oil Rodent Root Subcutaneous injection Tail Xylazine

Top 5 similar protocols

Variable analysis

independent variables

Subcutaneous injection of 20 µL of complete Freund's adjuvant (CFA; consisting of heat-killed Mycobacterium tuberculosis suspended in paraffin oil, 1 mg/mL) into the plantar surface of the right hind paw and to the tail root
Additional tail root injection of the same volume on Day 1

dependent variables

Inflammation of the right tibiotarsal joint

control variables

Anesthesia (i.p. ketamine (100 mg/kg) and xylazine (5 mg/kg))

controls

Positive control: CFA-induced arthritis, which is a well-established rodent model of chronic arthritis
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!