Immunoprecipitation from MDA-MB-231, A431 or H1299-R273H cytoplasmic extracts was performed as previously described.55 (link) For each IP, 2.0 μg of anti-p53 (D0–1, Santa Cruz, Santa Cruz, CA, USA), anti-p63 (H-137, Santa Cruz), anti-p73 (Bethyl, Montgomery, TX, USA), anti-Ago2 (Mouse 2A8), rabbit IgG, mouse IgG and anti-HA beads was used. ChIP assays (Primer sequences in Supplementary Table S9 ) were performed as described previously.32 (link)
Protocol detail
Immunoprecipitation and ChIP Assays
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Corresponding Organization :
Other organizations : National Institutes of Health, Howard University, National Cancer Institute, University of Mississippi Medical Center
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Variable analysis
independent variables
- Immunoprecipitation from MDA-MB-231, A431 or H1299-R273H cytoplasmic extracts
- ChIP assays
- 2.0 μg of anti-p53 (D0–1, Santa Cruz, Santa Cruz, CA, USA), anti-p63 (H-137, Santa Cruz), anti-p73 (Bethyl, Montgomery, TX, USA), anti-Ago2 (Mouse 2A8), rabbit IgG, mouse IgG and anti-HA beads
- Positive control: Not explicitly mentioned.
- Negative control: Rabbit IgG, mouse IgG
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