Evaluating HELF Migration and Healing

To evaluate the migratory/healing capacity of HELFs, cells were seeded in a 24-well plate (Ibidi, Gräfelfing, Germany). When HELFs were 90% confluent, stimulation/inhibition studies were performed and healing was evaluated after 20 h of incubation [33 (link),36 (link)]. Assessment of wound healing was achieved with both immunofluorescence and May–Grünwald–Giemsa (MGG) stain. Assay was performed by following the manufacturer’s instructions and recommendations.

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Ntinopoulou M., Cassimos D., Roupakia E., Kolettas E., Panopoulou M., Mantadakis E., Konstantinidis T, & Chrysanthopoulou A. (2023). Ιnterleukin-17A-Enriched Neutrophil Extracellular Traps Promote Immunofibrotic Aspects of Childhood Asthma Exacerbation. Biomedicines, 11(8), 2104.

Publication 2023

24-well plate

Assay Cells Immunofluorescence Inhibition May grünwald giemsa stain

Corresponding Organization : University of Ioannina

Other organizations : Democritus University of Thrace, Foundation for Research and Technology Hellas

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Variable analysis

independent variables

Stimulation/inhibition studies

dependent variables

Healing of HELFs
Wound healing assessment using immunofluorescence and May–Grünwald–Giemsa (MGG) stain

control variables

Seeding of HELFs in a 24-well plate
HELFs reaching 90% confluency
Incubation time of 20 hours

controls

Positive control: Not specified
Negative control: Not specified

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