Melanin Extraction from Antarctic Fungus

Melanin was extracted from dried colonies of C. antarcticus grown on different substrata, following the protocol optimized for black fungi [31 (link)]. After the extraction, purified pigments were dissolved in 500 µL of NaOH 1M and its UV-visible spectrum was measured in VWR-UV 1600 PC Spectrophotometer by using M.Wave professional 2.0 software (VWR, Radnor, Pennsylvania, USA). A standard graph for estimation was used and was made using synthetic melanin. NaOH 1M was used as a blank and the instrument was set in a range of 200–800 nm for the analysis. The correlation between absorbance and wavelength was defined. To determine the concentration of extracted melanin, synthetic DHN (1,8-DiHydroxyNaphthalene) melanin (Thermo Fisher Scientific) was prepared in 1M NaOH at concentrations of 500 mg/mL and a standard curve at 650 nm was obtained as reported in [32 (link)].

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Pacelli C., Cassaro A., Aureli L., Moeller R., Fujimori A, & Onofri S. (2020). The Responses of the Black Fungus Cryomyces Antarcticus to High Doses of Accelerated Helium Ions Radiation within Martian Regolith Simulants and Their Relevance for Mars. Life, 10(8), 130.

Publication 2020

VWR-UV 1600 PC Spectrophotometer synthetic DHN (1,8-DiHydroxyNaphthalene) melanin

Dihydroxynaphthalene melanin Fungi Melanin Pigments

Corresponding Organization : Università degli Studi della Tuscia

Other organizations : Agenzia Spaziale Italiana, Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR), Hochschule Bonn-Rhein-Sieg

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Variable analysis

independent variables

Substrata on which C. antarcticus was grown

dependent variables

UV-visible spectrum of extracted and purified pigments
Concentration of extracted melanin

control variables

NaOH 1M used as a blank for UV-visible spectrum measurements
Wavelength range of 200–800 nm used for UV-visible spectrum analysis

controls

Synthetic melanin (DHN, 1,8-DiHydroxyNaphthalene) used to prepare a standard curve for estimating the concentration of extracted melanin

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