miRNAs contained in sEVs from aPL, PAPS and HD (n = 8) were extracted following the instructions of miRCURY RNA Isolation Kit—Cell & Plant (Exiqon, Woburn, MA, USA). miRNA samples were processed and sequenced (single end, 50 nts, 1 × 50, v4) by CNAG (Barcelona, Spain) on a HiSeq2500 Illumina device with a read depth of >10 M reads/sample. Subsequent quality control, data processing and analysis were performed as previously described [48 (link),49 (link),50 (link)]. Data were deposited in the Gene Expression Omnibus (NCBI) with the number GSE220791 (more details in Supplementary Materials ).
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