Drosophila Genetic Screen for Physiological Traits

The Drosophila Genetics Reference Panel (DGRP) collection of wild type inbred sequenced strains (Mackay et al. 2012 (link); Huang et al. 2014 (link)) was used in all screens assessing physiological traits (fecundity, survival, intestinal regeneration, fecal motility, fecal spots, tracheal branching). The Vienna Drosophila Research Center (VDRC) UAS-RNAi lines used in this study were the following: 32863/GD and 106488/KK targeting CG42318/app, 45054/GD and 108473/KK targeting CG8348/Dh44, 19172/GD and 103958/KK targeting CG17330/jhamt, 13550/GD and 110349/KK targeting CG13654, 14641/GD and 101680/KK targeting CG43902, 14747/GD and 102995/KK targeting CG33344/CCAP-R, 37261/GD and 107921/KK targeting CG32058/Ir67c, 24275/GD and 110170/KK targeting CG8282/Snx6, 24746/GD and 103712/KK targeting CG3326/Fign, 13326/GD and 101365/KK targeting CG43946/Glut1. Other stocks used in this study were the following (source and/or stock center numbers in parentheses): UAS-apc^RNAi (VDRC, #1333), UAS-srcGFP (BDSC, #5432), Dl-Gal4 (Zeng et al. 2010 (link)), yw; ey-FLP2/T(2;3)CyO;TM6B,Tb,Hu (BDSC, #8204), Actin5C-Gal4 (BDSC, #25374).

Free full text: Click here

Evangelou A., Ignatiou A., Antoniou C., Kalanidou S., Chatzimatthaiou S., Shianiou G., Ellina S., Athanasiou R., Panagi M., Apidianakis Y, & Pitsouli C. (2019). Unpredictable Effects of the Genetic Background of Transgenic Lines in Physiological Quantitative Traits. G3: Genes|Genomes|Genetics, 9(11), 3877-3890.

Publication 2019

UAS-srcGFP Actin5C-Gal4

Ccap Center lines Drosophila Fecal Fecundity Glut1 Intestinal Motility Physiological Regeneration Rnai Spots Strains Tracheal

Corresponding Organization :

Other organizations : University of Cyprus

Top 5 similar protocols

Variable analysis

independent variables

Knockdown of CG42318/app
Knockdown of CG8348/Dh44
Knockdown of CG17330/jhamt
Knockdown of CG13654
Knockdown of CG43902
Knockdown of CG33344/CCAP-R
Knockdown of CG32058/Ir67c
Knockdown of CG8282/Snx6
Knockdown of CG3326/Fign
Knockdown of CG43946/Glut1
Knockdown of apc

dependent variables

Fecundity
Survival
Intestinal regeneration
Fecal motility
Fecal spots
Tracheal branching

control variables

Wild type inbred sequenced strains from the Drosophila Genetics Reference Panel (DGRP) collection
UAS-RNAi lines from the Vienna Drosophila Research Center (VDRC)
Yw; ey-FLP2/T(2;3)CyO;TM6B,Tb,Hu (BDSC, #8204)
Actin5C-Gal4 (BDSC, #25374)

controls

Positive control: UAS-srcGFP
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!