The histological analysis was performed as described previously [49 (link)]. In brief, at the end of the experiment, muscle tissues were prepared from mice, fixed in 10% neutral buffered formalin, embedded in paraffin. After serial 6 µm thick sections, tissue sections were deparaffinized using hot water and stained with hematoxylin and eosin (Vector Laboratories Inc., Newark, CA, USA). H and E-stained tissue sections were observed under a light microscope (AmScope, T690C-PL, Irvine, CA, USA), and images were taken with a microscopic digital camera (AmScope, MU-1803, Irvine, CA, USA).
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