U-Omp19 samples were run under both non-reducing and reducing conditions by SDS-PAGE as described previously [18] (link). For WB, after being subjected to SDS-PAGE, samples were transferred onto a nitrocellulose membrane. A polyclonal rabbit anti-U-Omp19 serum followed by anti-rabbit IgG labeled with IRDye 680 (LI-COR Biosciences) were used to visualize the bands. Images were captured and documented with an Odyssey infrared image-scanner (LI-COR Biosciences).
Laura Darriba M., Castro C.P., Coria L.M., Bruno L., Laura Cerutti M., Otero L.H., Chemes L.B., Rasia R.M., Klinke S., Cassataro J, & Pasquevich K.A. (2022). A disordered region retains the full protease inhibitor activity and the capacity to induce CD8+ T cells in vivo of the oral vaccine adjuvant U-Omp19. Computational and Structural Biotechnology Journal, 20, 5098-5114.
Secondary antibody (anti-rabbit IgG labeled with IRDye 680)
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
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