mESCs were cultured on irradiated MEFs in N2B27 medium with 2i (PD0325901, 0.4 μM [Stemgent, San Diego, CA, USA] and CHIR99021, 3 μM [Stemgent]) and LIF (1000
u/ml) in tissue culture (TC) dishes pretreated with 7.5 µg/ml poly-L-ornithine (Sigma, St. Louis, MO, USA) and 5 µg/ml laminine (BD Biosciences, San Jose, CA,
USA) [26 (link)].
u/ml) in tissue culture (TC) dishes pretreated with 7.5 µg/ml poly-L-ornithine (Sigma, St. Louis, MO, USA) and 5 µg/ml laminine (BD Biosciences, San Jose, CA,
USA) [26 (link)].
