Protocol detail

Root Segmentation and Analysis Protocol

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The root segmentation performed using X-ray CT images was validated using traditional destructive root length analyses. After the second scan, the cores; were opened and the soil was washed through a 1 mm sieve to collect roots. The roots were stored in ethanol until scanned on a flatbed scanner (Epson Perfection V850 pro) at 1200 dpi. The analysis of root length was done using the Rhizovision Explorer (V. 2.0.3 Seethepalli et al., 2021 (link)).

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Lucas M., Nguyen L.T., Guber A, & Kravchenko A.N. (2022). Cover crop influence on pore size distribution and biopore dynamics: Enumerating root and soil faunal effects. Frontiers in Plant Science, 13, 928569.

Publication 2022

Perfection V850 pro

Ethanol Root

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Variable analysis

independent variables

Root segmentation technique using X-ray CT images

dependent variables

Root length

control variables

Traditional destructive root length analyses
Washing the soil through a 1 mm sieve to collect roots
Storing the roots in ethanol until scanning on a flatbed scanner (Epson Perfection V850 pro) at 1200 dpi
Analysis of root length using the Rhizovision Explorer (V. 2.0.3 Seethepalli et al., 2021)

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