Prostate Cancer Cell Line Characterization

The human prostate epithelial cell line RWPE-1, the embryonic kidney cell line HEK-293 T, and the prostate cancer cell lines PC-3, LNCAP,22RV1,IE8 and DU145 were all acquired from American Type Culture Collection (ATCC, Manassas, Virginia, USA). The culture conditions for all cell lines adhered to previously established protocols [19 (link), 20 (link)]. Rigorous quality control was maintained, with all cell lines testing negative for mycoplasma contamination. Additionally, to ensure the authenticity and purity of the cell lines, short tandem repeat (STR) authentication was performed, confirming no misidentification or contamination (verified by IGE Biotechnology, Guangzhou, Guangdong, China).For in vitro studies, PC3 or DU145 PCa cells, were stimulated with recombinant human IL-11 (Abcam,ab89887) or recombinant human IL-11RΑ (rhIL-11RΑ 10 ng/ml;Abcam,ab132575) for the indicated group, replacing culture medium and stimulus every 24 h.