Spinogenesis experiments were performed as previously described5 (link) with the exception that cells were treated on DIV20 and fixed 24 h after treatment on DIV21. The images were taken on a Nikon HCA Confocal microscope a with a 100x/NA 1.45 oil objective. DMSO and ketamine (10 μM) were used as vehicle and positive controls, respectively.
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Spinogenesis Assay with Ketamine
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Corresponding Organization : Multidisciplinary Association for Psychedelic Studies
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Variable analysis
independent variables
- Treatment (DMSO, ketamine 10 μM)
- Spinogenesis (measured at DIV21)
- Cells were treated on DIV20 and fixed 24 h after treatment on DIV21
- Images were taken on a Nikon HCA Confocal microscope with a 100x/NA 1.45 oil objective
- DMSO (vehicle control)
- Ketamine 10 μM (positive control)
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