The xCELLigence system (ACEA Biosciences, Inc. San Diego, CA) was used for quantifying changes in real-time cell proliferation in NGP and SK-N-BE(2) cells as previously described.8 (link),9 (link) IMR-32 cells exhibited clumping when cultured, thus, proliferation of IMR-32 cells was determined by counting cell number daily using a Z1 Coulter Counter (Beckman Coulter, Indianapolis, IN) as previously described.26 (link)
Protocol detail
Real-time Cell Proliferation Assay
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Cells
Cells proliferation
Corresponding Organization : Pennsylvania State University
Other organizations : National Cancer Institute
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Variable analysis
independent variables
- Cell lines: NGP, SK-N-BE(2), and IMR-32
- Real-time cell proliferation for NGP and SK-N-BE(2) cells
- Cell number for IMR-32 cells
- Culture conditions for NGP and SK-N-BE(2) cells
- Culture conditions for IMR-32 cells
- No positive or negative controls were explicitly mentioned.
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