RNA interference was done as described
previously.45 (link) Briefly, siRNA for caspase-8,
-9, and -3 was purchased from Dhamacon Research, Inc. Nontargeting
control siRNA was purchased from Ambion. Transfections were performed
using Lipofectamine RNAiMAX (Invitrogen) in the reverse manner according
to the manufacturer’s instructions. Between 5 and 10 pmol of
siRNA and 5 μL of Lipofectamine RNAiMAX were mixed in each well
of 6-well plates for 20 min, followed by culturing 3 × 105 cells in the siRNA mix for 24–48 h; knockdown efficacy
was assessed by Western blotting.
previously.45 (link) Briefly, siRNA for caspase-8,
-9, and -3 was purchased from Dhamacon Research, Inc. Nontargeting
control siRNA was purchased from Ambion. Transfections were performed
using Lipofectamine RNAiMAX (Invitrogen) in the reverse manner according
to the manufacturer’s instructions. Between 5 and 10 pmol of
siRNA and 5 μL of Lipofectamine RNAiMAX were mixed in each well
of 6-well plates for 20 min, followed by culturing 3 × 105 cells in the siRNA mix for 24–48 h; knockdown efficacy
was assessed by Western blotting.
