JAr EVs for
TEM imaging were
prepared as described before46 (link) with modifications.
Briefly, 20 μL of the EV suspension was deposited on formvar-carbon-coated
200 mesh copper grids (Agar Scientific, Essex, U.K.) for 20 min and
fixed in Karnowski fixatives (a mixture of 2% paraformaldehyde and
1% glutaraldehyde) for 5 min. Next, EVs were contrasted for 5 min
in uranyl oxalate [1:1 mixture of 4% uranyl acetate (21447-25, Polysciences,
Warrington) and 0.15 M oxalic acid (75688, Sigma-Aldrich, Schnelldorf,
Germany)] and embedded in a mixture (9:1) of 2% methylcellulose (M6385,
Sigma-Aldrich, Schnelldorf, Germany) and 4% uranyl acetate (21447-25,
Polysciences, Warrington). The EVs were imaged using a JEM 1400 TEM
(JEOL Ltd. Tokyo, Japan, with Morada TEM CCD camera, Olympus, Germany)
at 80 kV.
TEM imaging were
prepared as described before46 (link) with modifications.
Briefly, 20 μL of the EV suspension was deposited on formvar-carbon-coated
200 mesh copper grids (Agar Scientific, Essex, U.K.) for 20 min and
fixed in Karnowski fixatives (a mixture of 2% paraformaldehyde and
1% glutaraldehyde) for 5 min. Next, EVs were contrasted for 5 min
in uranyl oxalate [1:1 mixture of 4% uranyl acetate (21447-25, Polysciences,
Warrington) and 0.15 M oxalic acid (75688, Sigma-Aldrich, Schnelldorf,
Germany)] and embedded in a mixture (9:1) of 2% methylcellulose (M6385,
Sigma-Aldrich, Schnelldorf, Germany) and 4% uranyl acetate (21447-25,
Polysciences, Warrington). The EVs were imaged using a JEM 1400 TEM
(JEOL Ltd. Tokyo, Japan, with Morada TEM CCD camera, Olympus, Germany)
at 80 kV.
