The procedure and analysis of the CSF followed the Alzheimer's Association Flow Chart for CSF biomarkers.7 Lumbar CSF samples were collected at the three centers and analyzed according to a standardized protocol.7 , 20 CSF Aβ42, Aβ40, and Aβ38 were analyzed by Euroimmun (EI) (EUROIMMUN AG, Lübeck, Germany) and Meso Scale Discovery (MSD) (Rockville, MD) immunoassays. CSF Aβ40 and Aβ42 were also analyzed using Quanterix (Quanterix, Lexington, MA) immunoassay. The EI assays were used to measure CSF Aβ (1–42, 1–40, and 1–38) in all three cohorts. In addition, in cohort‐1, all the CSF samples were analyzed with MSD kit (specific for AβN‐42, AβN‐40 and AβN‐38) and 70 CSF samples were analyzed using Quanterix kit (specific for AβN‐42 and AβN‐40).
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Standardized CSF Biomarker Analysis
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Corresponding Organization : Skåne University Hospital
Other organizations : University of Gothenburg, National Hospital for Neurology and Neurosurgery, University College London
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Variable analysis
independent variables
- Procedure and analysis of the CSF
- Immunoassays used to analyze CSF Aβ42, Aβ40, and Aβ38 (Euroimmun, Meso Scale Discovery, Quanterix)
- Levels of CSF Aβ42, Aβ40, and Aβ38
- Standardized protocol for CSF sample collection and analysis across the three centers
- None explicitly mentioned
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