An enzyme-linked indirect immunosorbent assay (ELISA) was performed on sera samples for the detection of specific antibodies of L. infantum (INGEZIM® Leishmania; Ingenasa, Madrid, Spain), Ehrlichia canis (INGEZIM® Ehrlichia; Ingenasa) and Anaplasma phagocytophilum (Anaplasma-ELISA Dog; AFOSA GmbH, Blankenfelde-Mahlow, Germany). All assays were performed following the respective manufacturer’s instructions. All sera samples collected on all SDs were tested for all of the above pathogens.
Ligda P., Gizzarelli M., Kostopoulou D., Foglia Manzillo V., Saratsis A., Saratsi K., Michler S., Ringeisen H., Boegel A., Schunack B., Pollmeier M., Kontrafouris M., Tsatsaki O., Oliva G, & Sotiraki S. (2023). Determination of the effect of collars containing 10% w/w imidacloprid and 4.5% w/w flumethrin (Seresto®) on the incidence of Leishmania and other canine vector-borne pathogen infections in Greece. Parasites & Vectors, 16, 89.
Detection of specific antibodies of Ehrlichia canis
Detection of specific antibodies of Anaplasma phagocytophilum
control variables
All sera samples collected on all SDs were tested for all of the above pathogens
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
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