Investigating Interferon Response Pathway Using qPCR
RNA extraction was performed using the RNeasy Kit (Qiagen) per the manufacturer’s protocol. Reverse transcription was performed using qScript cDNA SuperMIx (Quantabio). qPCR analysis was performed using TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) on the Roche Light Cycler 480. TaqMan Gene Expression Assays of IFIT1 (Hs03027069_s1), IFIT2 (Hs01922738_s1), IFIT3 (Hs01922752_s1), IRF1 (Hs00971965_m1), CXCL9 (Hs00970538_m1), CXCL10 (Hs00171042_m1), CXCL11 (Hs00171138_m1), DUSP6 (Hs04329643_s1), ETV4 (Hs00383361_g1), ETV5 (Hs00927578_g1) and SPRY4 (Hs01935412_s1) were purchased from Thermo Fisher Scientific. B-actin (Thermo Fisher Scientific; 4326315E) was used as endogenous control.
Tian J., Chen J.H., Chao S.X., Pelka K., Giannakis M., Hess J., Burke K., Jorgji V., Sindurakar P., Braverman J., Mehta A., Oka T., Huang M., Lieb D., Spurrell M., Allen J.N., Abrams T.A., Clark J.W., Enzinger A.C., Enzinger P.C., Klempner S.J., McCleary N.J., Meyerhardt J.A., Ryan D.P., Yurgelun M.B., Kanter K., Van Seventer E.E., Baiev I., Chi G., Jarnagin J., Bradford W.B., Wong E., Michel A.G., Fetter I.J., Siravegna G., Gemma A.J., Sharpe A., Demehri S., Leary R., Campbell C.D., Yilmaz O., Getz G.A., Parikh A.R., Hacohen N, & Corcoran R.B. (2023). Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trial. Nature Medicine, 29(2), 458-466.
Other organizations :
Massachusetts General Hospital, Harvard University, Broad Institute, Massachusetts Institute of Technology, Dana-Farber Cancer Institute, Boston VA Research Institute, Novartis (United States)
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