Lung tissue sections were stained using anti-FOXM-1, anti-Ki-67, anti-PH3 (Santa Cruz) and anti-Cleaved Caspase-3 (Abcam) antibodies, as described previously (33 (link)). Tumor cells growing on coverslips were treated with 20 μM of RCM-1 for 24 h, fixed and stained with antibodies against FOXM1, FOXA1, β-catenin, Ki-67 and α-tubulin (Santa Cruz) as previously described (32 (link)).
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Immunohistochemical Analysis of Cell Proliferation and Apoptosis
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Corresponding Organization : Cincinnati Children's Hospital Medical Center
Other organizations : University of Cincinnati, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
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Variable analysis
independent variables
- Treatment with 20 μM of RCM-1 for 24 h
- Expression levels of FOXM1, FOXA1, β-catenin, Ki-67 and α-tubulin in tumor cells
- Immunohistochemical staining of lung tissue sections for FOXM-1, Ki-67, PH3, and Cleaved Caspase-3
- Tumor cells growing on coverslips
- Lung tissue sections
- Positive control: No positive control mentioned
- Negative control: No negative control mentioned
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