Extraction of Crude Polysaccharide from D. indusiata

The extraction of crude polysaccharide from the fruiting body of the mushroom D. indusiata was done following the procedure reported previously [55 (link)]. Briefly, the fruiting bodies of D. indusiata were dried in a hot air-drying oven at 45 °C and crushed into powder using a tissue triturator. The powder of fruiting body was extracted by using boiling water and the collected supernatant was then concentrated at 60 °C using a rotary evaporator. After that, the concentrated supernatant was deproteinated several times by the Sevag method [56 ] and the protein content was determined by the bicinchoninic acid (BCA) protein assay kit (Pierce, Rockford, IL, USA) following the manufacturer’s instructions. The resulting solution was ethanol precipitated, dried using vacuum freeze drying and collected as a crude polysaccharide (DIP).