Migration assays were performed as described previously (19 (link)). Briefly, cells (5 × 104) were plated in medium without serum in the upper well of a transwell chamber of a 24-well transwell with 8-μm pores (BD Biosciences), placed in a bottom chamber containing medium supplemented with 10% fetal bovine serum. After 48 h, the membranes were fixed and stained with haematoxylin solution, and migration was quantified by counting ten random fields under a light microscope (400×). The mean value was calculated from data obtained from three separate chambers.
Protocol detail
Transwell Cell Migration Assay
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Shantou University Medical College
Other organizations : University of Pennsylvania, Shantou Central Hospital, Cedars-Sinai Medical Center
Top 5 similar protocols
Variable analysis
independent variables
- Cell seeding density (5 × 104 cells)
- Cell migration (measured by counting the number of cells that migrated through the membrane)
- Transwell chamber with 8-μm pores
- Medium without serum in the upper well
- Medium supplemented with 10% fetal bovine serum in the bottom chamber
- Incubation time (48 h)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!