Retrograde Labeling of Retinal Ganglion Cells

Mice were anesthetized with chloral hydrate and placed in a stereotactic apparatus. A 1 μl volume of 4% Fluoro-Gold (FG, Invitrogen) suspension was injected into the superior colliculi as previously described^{51 (link), 52}. Seven days were allotted for the retrograde transport of FG to label RGCs. After the experiment, a retinal wholemount was prepared, and FG positive RGCs were identified under a confocal fluorescence microscope (LSM710, Carl Zeiss).

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Zhang L., Xu J., Liu R., Chen W., Chen Q., Hu W., Zhou L., Zhang R., Xu H., Lin D., Li X, & Tang Z. (2017). Caveolin-1 Protects Retinal Ganglion Cells against Acute Ocular Hypertension Injury via Modulating Microglial Phenotypes and Distribution and Activating AKT pathway. Scientific Reports, 7, 10716.

Publication 2017

Fluoro-Gold (FG, LSM710

Chloral hydrate Fluorescence microscope Fluoro gold Mice Retinal Superior colliculi

Corresponding Organization :

Other organizations : Sun Yat-sen University

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Variable analysis

independent variables

Injection of 4% Fluoro-Gold (FG) suspension into the superior colliculi

dependent variables

Retrograde transport of FG to label retinal ganglion cells (RGCs)
Identification of FG positive RGCs under a confocal fluorescence microscope

control variables

Anesthesia with chloral hydrate
Placement of mice in a stereotactic apparatus
Allotment of 7 days for retrograde transport of FG
Preparation of retinal wholemount

controls

No positive or negative controls were explicitly mentioned in the input text.

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