Fluorescence Microscope Imaging System

The imaging system consists of an inverted fluorescence microscope (IMT-2, Olympus), mounted with a Plan-Apochromat 20× /0.8NA objective (Carl Zeiss) and a fluorescence filter cube; a Royal-Blue LED (Luxeonstar) served for the fluorophore excitation. A CCD (GX1920, Allied Vision Technologies) was mounted via zoom and 0.1× c-mount adaptors (Vario-Orthomate 543513 and 543431, Leitz), sampling at 70 Hz, 968 × 728 px, covering 810 × 610 μm laterally and the full depth of the tube. The camera control was based on a modification of the Motmot Python camera interface package^{35 (link)}, expanded with a home-made plug-in, to allow real-time image analysis in the RAM^{23 (link)}, recording only the time-lapse positions of the tracers to the hard drive.

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Afik E, & Steinberg V. (2017). On the role of initial velocities in pair dispersion in a microfluidic chaotic flow. Nature Communications, 8, 468.

Publication 2017

IMT-2 Plan-Apochromat 20× /0.8NA objective

Fluorescence Fluorescence microscope Python Vision

Corresponding Organization :

Other organizations : Weizmann Institute of Science

Top 5 similar protocols

Variable analysis

independent variables

Fluorescence filter cube
Royal-Blue LED (Luxeonstar) for fluorophore excitation

dependent variables

Time-lapse positions of the tracers

control variables

Inverted fluorescence microscope (IMT-2, Olympus)
Plan-Apochromat 20× /0.8NA objective (Carl Zeiss)
CCD (GX1920, Allied Vision Technologies) with 70 Hz sampling rate, 968 × 728 px resolution, covering 810 × 610 μm laterally and the full depth of the tube
Camera control based on a modification of the Motmot Python camera interface package, expanded with a home-made plug-in to allow real-time image analysis in the RAM

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