C3H10T1/2 cells were plated on 96-well cell culture plates (2 × 104 cell/well) in 100 μL of medium and grown for 24 h at 37 °C. Thereafter, RSG 1 µM was added with or without EPA 10 µM and/or DHA 10 µM and the cells were incubated for another 72 h. Cell proliferation was evaluated using an MTS proliferation assay kit (Promega Inc., USA)57 (link).
Free full text: Click here