Matrigel Invasion Assay

The invasion assay was performed in 24-well plate for 20 h. The transfected cells (3 × 10⁴) in 200 μL of serum free medium were seeded onto upper Matrigel matrix (Corning, New York, NY, USA) coated Cell Culture Insert (Greiner Bio One, Kremsmünster, Austria). The lower chamber contained 900 μL of complete culture medium. The invaded cells were fixed with methanol for 10 min and stained with 0.005% crystal violet for 1 h at room temperature. The numbers of invaded cells were counted under the microscope from 10 random fields^{55 (link)}.

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Huang Y.H., Chu P.Y., Chen J.L., Huang C.T., Huang C.C., Tsai Y., Wang Y.L., Lien P.J., Tseng L.M, & Liu C.Y. (2021). Expression pattern and prognostic impact of glycoprotein non-metastatic B (GPNMB) in triple-negative breast cancer. Scientific Reports, 11, 12171.

Publication 2021

Matrigel matrix Cell Culture Insert

Assay Cell culture Cells Crystal violet Culture medium Matrigel Methanol Microscope Serum

Corresponding Organization :

Other organizations : National Yang Ming Chiao Tung University, Chang Bing Show Chwan Memorial Hospital, Taipei Veterans General Hospital

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Variable analysis

independent variables

Transfected cells

dependent variables

Number of invaded cells

control variables

Serum-free medium
Matrigel matrix coated Cell Culture Insert
Complete culture medium in the lower chamber
Methanol fixation
Crystal violet staining

controls

Positive control: Not mentioned
Negative control: Not mentioned

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