p62/SQSTM1 Knockdown and Autophagy Regulation

A set of p62/SQSTM11 shRNA vectors (SHCLNG-NM_003900), and a non-target shRNA control vector were purchased from Sigma-Aldrich (St. Louis, MO) for the p62/SQSTM1 knockdown experiments. Lentivirus production and viral infection were done as previously described [23] (link). Small interfering RNA (siRNA) duplexes were purchased from Ambion (Austin, TX) targeting ATG7 (ID# s20650 and s20651). Negative Control #1 siRNA was used as control. siRNAs (30 µM per 100 µl transfection solution) were electroporated using Lonza's Nucleofector and the Cell Line Neucleofection kit V (Walkersville, MD) As described in [23] (link)