Real-time RT-PCR analyses were performed described previously [68 (link)]. Briefly, total RNA from RWPE-1 cells were isolated using a QIAzol lysis reagent (QIAGEN sciences Inc., Venlo, Netherlands) and a GeneAllR RiboEx Total RNA extraction kit (GeneAll Biotechnology, Seoul, Korea). Total RNA was used as a template for first strand cDNA synthesis by a Power cDNA synthesis kit (iNtRON Biotechnology, Seoul, Korea). Step One Plus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) was used for PCR analysis. The primers for Srd5ar2 were Forward 5′ TCC CGC TTG GCC TTT TG-3′ and Reverse 5′- GCC GTT ACC CTC CTT GTT TTC-3′.
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